Microscopy Software - Entry Level Image Analysis

Clemex Captiva’s robust annotation and direct measure tool set includes many properties and variables. An image can contain a corporate logo and comments in addition to measurements such as perimeters, areas, or angles. Graphs are automatically generated and data is compiled in a browser. Images and data can be exported to MS Office programs for easy reporting customized to your needs.

Uneven illumination is often a source of poor image quality. Clemex Captiva’s Shading Corrector eliminates these adverse effects to produce a high quality image. Applied on the live image, the Shading Corrector allows even lighting for every field of the sample ensuring seamless mosaic images.

Before applying shading corrector		After applying shading corrector

With the Clemex Mosaic feature, Clemex Captiva automatically stitches multiple fields to form a large image of unlimited resolution. This feature can be used either manually or with a motorized stage for added efficiency.

Images captured at 100X		Perfect stitching 2x3 composite image. Total file size: 7.89Mb

Manual Mode
The manual mode is used to make small mosaics (i.e. 6 fields). The software tracks the pattern match (green line in figure 2 & 3) as the operator moves the stage manually. When the line turns red (figure 4), a snapshot is taken before moving to the next field. The image is then stitched together automatically (figure 5). Using Multi-Layer Grabs allow for a perfect X, Y, and Z focused image.

fig.1	fig.2
fig. 4	fig. 3	fig.5

Automatic Mode with Motorized Stage
The automatic mode uses the motorized stage to make large Mosaics and Multi-Layer Grabs. The whole operation is mechanized; simply enter the parameters at the outset, click and walk away while the system detects the pattern matches in real time. The Mosaic function and the Multi-Layer Grabs work jointly to provide a sharp image of the
sample.

Calibration, the process of assigning a size to a pixel, is an essential operation for any image analysis. The Clemex NIST traceable micrometer helps users assign a different calibration setting for each objective. With a motorized stage, simply click on Calibration and follow the instructions when prompted. Without a motorized stage, stretch the caliper over the micrometer and enter the value in the dialog box. It’s easy and returns accurate, reproducible, and certified results that can be validated anytime.